So I did another egg count just now, my second since a few months ago. I followed these instructions: http://www.docstoc.com/docs/599020/Macmaster-counting-Technique-ppt except rather than making a large volume of flotation fluid, I mixed 40 g of salt with 100 ml. of water. I wrote about the technique and where I got my supplies here.
Also filed in
|
|
Eureka! I finally managed to see some hookworm larvae under the microscope. For a full visual description of the McMaster egg count, see: http://www.docstoc.com/docs/599020/Macmaster-counting-Technique-ppt Results from 10 larvae? 800 epg I’ll walk through my steps one by one. First, I bought the materials: digital scale: myweigh ibalance 500 (more expensive then the cheaper models, but [...]
Also filed in
|
|
Saturday, February 28, 2009
I’m researching incubation methods, and hopefully in a few weeks I will embark on my first McMaster egg count. There seem to be 3 choices for incubating the hookworm larvae; the Baermann, Harada-Mori, and the AP method (agar plate.) Here’s a nice visual and thorough list of requirements for the first two methods: http://books.google.com/books?id=8AWz0cS6e9kC&pg=PA115&lpg=PA115&dq#PPA115,M1 This [...]
Wednesday, February 11, 2009
There are very few studies on the effect of helminths on humans. As a patient trying this therapy, there are few immune markers we have on hand to check immune response. We have measures of inflammation, like CRP and SED rates, but only in the research setting can one measure IL-10, the T 1 and [...]
I’ve been wondering why there are so many false negatives when using standard ova tests that are offered through such labs as Quest, etc. This study shows that even waiting 3 hours between stool sample and test allows for a 50% loss of eggs, so I wonder if the sample is over 24 hours old, [...]