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	<title>Waiting for the Cure &#187; hookworm lifecycle</title>
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	<link>http://waitingforthecure.com/I</link>
	<description>... a day in the life of Crohn's disease ...</description>
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		<title>incubation techniques for hookworm larvae</title>
		<link>http://waitingforthecure.com/I/2009/02/28/incubation-techniques-for-hookworm-larvae/</link>
		<comments>http://waitingforthecure.com/I/2009/02/28/incubation-techniques-for-hookworm-larvae/#comments</comments>
		<pubDate>Sat, 28 Feb 2009 15:50:36 +0000</pubDate>
		<dc:creator>I</dc:creator>
				<category><![CDATA[egg count]]></category>
		<category><![CDATA[hookworm lifecycle]]></category>
		<category><![CDATA[incubation methods]]></category>
		<category><![CDATA[hookworm]]></category>
		<category><![CDATA[necator americanus]]></category>

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		<description><![CDATA[I&#8217;m researching incubation methods, and hopefully in a few weeks I will embark on my first McMaster egg count. There seem to be 3 choices for incubating the hookworm larvae; the Baermann, Harada-Mori, and the AP method (agar plate.) Here&#8217;s a nice visual and thorough list of requirements for the first two methods: http://books.google.com/books?id=8AWz0cS6e9kC&#038;pg=PA115&#038;lpg=PA115&#038;dq#PPA115,M1 This [...]]]></description>
			<content:encoded><![CDATA[<p>I&#8217;m researching incubation methods, and hopefully in a few weeks I will embark on my first <a href="http://www.docstoc.com/docs/599020/Macmaster-counting-Technique-ppt">McMaster egg count</a>.  There seem to be 3 choices for incubating the hookworm larvae; the Baermann, Harada-Mori, and the AP method (agar plate.)  Here&#8217;s a nice visual and thorough list of requirements for the first two methods:</p>
<p><a href="http://books.google.com/books?id=8AWz0cS6e9kC&#038;pg=PA115&#038;lpg=PA115&#038;dq#PPA115,M1">http://books.google.com/books?id=8AWz0cS6e9kC&#038;pg=PA115&#038;lpg=PA115&#038;dq#PPA115,M1</a></p>
<p>This article goes into great detail on the advantages of each method:</p>
<p><a href="http://www.ajtmh.org/cgi/content/full/77/6/1087">http://www.ajtmh.org/cgi/content/full/77/6/1087</a></p>
<p>Incubation temperature is important, and 30C (86 degrees farenheit) is optimal: </p>
<p><a href="http://www.ncbi.nlm.nih.gov/pubmed/3569472">http://www.ncbi.nlm.nih.gov/pubmed/3569472</a></p>
<p>I&#8217;ll provide an equipment list and write about the process once I&#8217;ve ordered the supplies, and tried it out.  Oh why didn&#8217;t I take a science class in college?  An art major in oil painting is not helping me out here&#8230;perhaps I&#8217;ll start a larval triptych!</p>
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		<title>Columbia transcript on Hookworms</title>
		<link>http://waitingforthecure.com/I/2008/12/30/columbia-transcript-on-hookworms/</link>
		<comments>http://waitingforthecure.com/I/2008/12/30/columbia-transcript-on-hookworms/#comments</comments>
		<pubDate>Tue, 30 Dec 2008 16:04:05 +0000</pubDate>
		<dc:creator>I</dc:creator>
				<category><![CDATA[hookworm lifecycle]]></category>
		<category><![CDATA[studies]]></category>
		<category><![CDATA[hookworm necator americanus]]></category>

		<guid isPermaLink="false">http://waitingforthecure.com/I/?p=316</guid>
		<description><![CDATA[http://www.cumc.columbia.edu/dept/ps/2007/para/old/transcript_02_pd02.pdf A little more information about hookworms and the hookworm eradication project&#8230; Is this true about the males? That they detach after mating; I wonder how the life cycle would continue then.]]></description>
			<content:encoded><![CDATA[<p><a href="http://www.cumc.columbia.edu/dept/ps/2007/para/old/transcript_02_pd02.pdf">http://www.cumc.columbia.edu/dept/ps/2007/para/old/transcript_02_pd02.pdf</a></p>
<p>A little more information about hookworms and the hookworm eradication<br />
project&#8230;</p>
<p>Is this true about the males? That they detach after mating; I wonder<br />
how the life cycle would continue then.</p>
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